000			03549nam a22003615i 4500
001			235688
003			ES-VaUE
005			20221220020720.0
007			cr nn 008mamaa
008			100301s1997 xxu| s |||| 0|eng d
020			_a9781592595747
024	7		_a10.1385/089603447X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDrug'DNA Interaction Protocols _cedited by Keith R. Fox.
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (X, 278 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v90
505	0		_aDNase I Footprinting -- Quantitative DNA Footprinting -- Uranyl Photoprobing of DNA Structures and Drug-DNA Complexes -- Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro -- Footprinting Studies with Nucleosome-Bound DNA -- A Gel Mobility Shift Assay for Probing the Effect of Drug-DNA Adducts on DNA-Binding Proteins -- An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug-Binding Sites -- DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug-DNA Interactions -- Transcriptional Footprinting of Drug-DNA Interactions -- Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays -- PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells -- Analysis of DNA-Binding Antibodies -- Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies -- Optical Absorbance and Fluorescence Techniques for Measuring DNA-Drug Interactions -- Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves -- Electric Dichroism -- Calorimetric Techniques for Studying Drug-DNA Interactions -- Methods for the Studies of Drug Dissociation from DNA.
520			_aIn Drug-DNA Interactions, expert researchers describe powerful molecular techniques designed to illuminate and explore the interaction of drugs and ligands with DNA, often perfected in their own laboratories, . These easily reproducible methods involve sequence recognition properties, as well as physical approaches to measuring both the strength of interaction and the mode of drug binding to DNA. Drug interactions are also examined from a cellular perspective and for their usefulness in the development of new therapeutic agents. Drug-DNA Interactions will be highly useful to all investigators needing the kind of detailed experimental and technical information often omitted from scientific papers. Those wishing to expand their repertoire of experimental techniques or to improve their current techniques will also find these powerful methods particularly helpful in elucidating the action of existing therapeutic agents, as well as in designing new anticancer drugs with improved action and reduced toxicity.
700	1		_aFox, Keith R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942883
776	0	8	_iPrinted edition: _z9781489942876
776	0	8	_iPrinted edition: _z9780896034471
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/089603447X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1997
999			_c235688 _d235688