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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592111
024	7		_a10.1385/1592592112 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGenomic Imprinting _bMethods and Protocols _cedited by Andrew Ward.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XII, 377 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v181
505	0		_aGeneration of Monoparental Embryos for Investigation into Genomic Imprinting -- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting -- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome -- Production of YAC Transgenic Mice by Pronuclear Injection -- A Transgenic Approach to Studying Imprinted Genes -- Methylation-Sensitive Genome Scanning -- Subtraction-Hybridization Method for the Identification of Imprinted Genes -- Identification of Imprinted Loci by Methylation -- Ribonuclease Protection -- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression -- Allele-Specific In Situ Hybridization (ASISH) -- RNA-FISH to Analyze Allele-Specific Expression -- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes -- Southern Analysis Using Methyl-Sensitive Restriction Enzymes -- A PCR-Based Method for Studying DNA Methylation -- Bisulfite-Based Methylation Analysis of Imprinted Genes -- Direct Analysis of Chromosome Methylation -- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs -- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation -- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon -- Probing Chromatin Structure with Nuclease Sensitivity Assays -- Examining Histone Acetlylation at Specific Genomic Regions -- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis -- Reconstitution of Chromatin In Vitro -- Genomic Imprinting in Plants.
520			_aImprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Protocols, Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. The majority of protocols describe molecular techniques that allow examination of gene structure or expression in an allele-specific manner. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results. Incorporating a wealth of knowledge from leading exponents in the field, Genomic Imprinting: Methods and Protocols brings together all the essential molecular, genetic, and embryological methods commonly used in today's laboratories for the identification and analysis of imprinted genes.
700	1		_aWard, Andrew _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371646
776	0	8	_iPrinted edition: _z9780896037410
776	0	8	_iPrinted edition: _z9781489938954
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592112 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235689 _d235689