000			03523nam a22003615i 4500
001			235697
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591800
024	7		_a10.1385/1592591809 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHigh Throughput Screening _bMethods and Protocols _cedited by William P. Janzen.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (X, 265 páginas) _b31 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v190
505	0		_aDesign and Implementation of High Throughput Screening Assays -- Configuring Radioligand Receptor Binding Assays for HTS Using Scintillation Proximity Assay Technology -- Homogeneous Techniques for Monitoring Receptor-Ligand Interactions -- Comparison of SPA, FRET, and FP for Kinase Assays -- Electrochemiluminescence: A Technology Evaluation and Assay Reformatting of the Stat6/P578 Protein-Peptide Interaction -- Cellular Assays in HTS -- Compound Library Management -- Compound Library Management: An Overview of an Automated System -- Natural Products or Not? How to Screen for Natural Products in the Emerging HTS Paradigm -- to Screening Automation -- Unit Automation in High Throughput Screening -- Fully Automated Screening Systems -- Management and Maintenance of High Throughput Screening Systems.
520			_aSince its first use in antibiotic research in the 1940s, High Throughput Screening (HTS) has evolved into a major enabling tool and methodology in the discovery of pharmaceutical, chemical, and agricultural compounds. In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wished had been available when they started in HTS, these seasoned authors illuminate the HTS process with richly detailed tutorials on the biological techniques involved, the management of compound libraries, and the automation and engineering approaches needed. Extensive discussions provide readers with all those key elements of pharmacology, molecular biology, enzymology, and biochemistry that will ensure the identification of suitable targets and screens, and detail the technology necessary to mine millions of data points for meaningful knowledge. By presenting accounts not only of the basic principles underlying HTS technology, but also real-use cases, High Throughput Screening constitutes a highly practical introduction to HTS, even as it also provides sufficient detail to work more productively in today's lead generation laboratories.
700	1		_aJanzen, William P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489938688
776	0	8	_iPrinted edition: _z9780896038899
776	0	8	_iPrinted edition: _z9781489938671
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591809 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235697 _d235697