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020 _a9781592596706
024 7 _a10.1385/1592596703
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHepatitis B and D Protocols
_bVolume 2: Immunology, Model Systems, and Clinical Studies
_cedited by Robert K. Hamatake, Johnson Y. N. Lau.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XXIII, 572 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v96
505 0 _aViral-Specific Immunological and Other Host Responses -- Studying Host Immune Responses Against Duck Hepatitis B Virus Infection -- Measurement of Cell-Mediated Immune Response in Woodchucks -- Study of Liver-Specific Expression of Cytokines During Woodchuck Hepatitis Virus Infection -- Induction of Anti-Hepatitis B Virus Immune Responses Through DNA Immunization -- Monitoring Gene Expression Using DNA Microarrays During Hepatitis B Virus Infection -- Determination of Hepatitis B Virus-Specific CD8+ T-Cell Activity in the Liver -- Determining the Precursor Frequency of HBV Nucleocapsid Antigen-Specific T Cells -- Detection and Characterization of Virus-Specific CD8+ T Cells Using the Tetramer Approach -- In Vitro Analysis of Hepatitis B Virus Specific CD4+ T Cells -- Induction of Humoral and Cellular Immune Responses to Hepatitis Delta Virus Through DNA Immunization in BALB/c Mice -- In Vitro and In Vivo Models -- Infection of Primary Chimpanzee Hepatocytes with Recombinant Hepatitis D Virus Particles -- Study of the Endocytosis and Intracellular Localization of Subviral Particles of Hepatitis B Virus in Primary Hepatocytes -- The Tupaia Model for the Study of Hepatitis B Virus -- Delivery of Hepatitis B Virus Therapeutic Agents Using Asialoglycoprotein Receptor-Based Liver-Specific Targeting -- Woodchuck Hepatitis Virus Hepatocyte Culture Models -- Duck Hepatitis B Virus Primary Hepatocyte Culture Model -- Enhancement of Infection of HepG2 Cells in Culture by Predigestion of Hepadnavirus with V8 Protease -- Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus -- Baculovirus-Mediated Gene Transfer for the Study of Hepatitis B Virus -- Transgenic Hepatitis B Virus Mouse Model in the Study of Chemotherapy -- Transplantation of Human Hepatocytes in Immunodeficient UPA Mice -- Duck Hepatitis B Virus Model in the Study of Hepatitis B Virus -- Hepatitis B Virus Transgenic Severe Combined Immunodeficient Mouse Model of Acute and Chronic Liver Disease -- The Chimpanzee Model -- Hepatitis B in Liver Transplant Recipients as a Special Model of Antiviral Drug Development -- Hepatitis Delta Virus Transfection for the Mouse In Vivo Model -- Hepatitis Delta Virus RNA Transfection for the Cell Culture Model -- Antiviral Testing and Clinical Studies -- Analysis of Hepatitis B Virus Dynamics and Its Impact on Antiviral Development -- Genotyping Anti-Hepatitis B Virus Drug Resistance -- A Parsimonious Method for Screening Drug Combinations for Antihepadnaviral Activity Using a Parametric Dose-Response Surface Approach -- Hepatitis B Virus -- Designing Studies to Evaluate Anti-Hepatitis B Virus Therapies -- Specific Considerations in the Design of Hepatitis B Virus Clinical Studies in the Far East -- Studying the Treatment of Chronic Hepatitis B Viral Infection in Special Populations -- Designing Clinical Development Programs for Anti-Hepatitis B Virus Drugs -- Testing Antivirals Against Hepatitis Delta Virus.
520 _aDespite the availability of an effective vaccine, there are still 400 million people worldwide who are chronically infected with the hepatitis B and D viruses (HBV and HDV), and more work still needs to be done in all of the key areas of managing the disease. In Hepatitis B and D Protocols, leading investigators and clinicians have joined forces to create a broad-ranging collection of cutting-edge techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this second of two volumes, Immunology, Model Systems, and Clinical Studies, the authors focus on protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying first volume, Detection, Genotypes, and Characterization, contains user-friendly protocols for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Taken together, Hepatitis B and D Protocols, Volume 1: Detection, Genotypes, and Characterization and Volume 2: Immunology, Model Systems, and Clinical Studies offer both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, as well as an essential resource for finding new therapies to treat chronically infected patients.
700 1 _aHamatake, Robert K
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aLau, Johnson Y. N
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617373633
776 0 8 _iPrinted edition:
_z9781588291080
776 0 8 _iPrinted edition:
_z9781489943774
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596703
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c235709
_d235709