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020 _a9781493926688
024 7 _a10.1007/978-1-4939-2668-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHuman Embryonic Stem Cell Protocols
_cedited by Kursad Turksen.
250 _a3rd edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XIV, 399 páginas)
_b68 ilustraciones, 40 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1307
505 0 _aDerivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts -- Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel -- Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix -- Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate -- Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Study of Gap Junctions in Human Embryonic Stem Cells -- Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System -- Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells -- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation -- Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells -- Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts -- Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa -- Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections -- Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering -- Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells -- Directed Differentiation of Human Embryonic Stem Cells into Neural Progenitors -- Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives -- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small Molecule Inhibitor of Src Family Kinases -- Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells -- Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium -- Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells -- Generation of Megakaryocytes and Platelets from Human Pluripotent Stem Cells -- A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.
520 _aThe potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.   Thorough and practical, Human Embryonic Stem Cell Protocols, Third Edition serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting.
700 1 _aTurksen, Kursad
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493926671
776 0 8 _iPrinted edition:
_z9781493926695
776 0 8 _iPrinted edition:
_z9781493949984
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2668-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2016
999 _c235711
_d235711