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001			235712
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007			cr nn 008mamaa
008			161213s2017 xxu| s |||| 0|eng d
020			_a9781493967438
024	7		_a10.1007/978-1-4939-6743-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEpigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases _cedited by Barbara Stefanska, David J. MacEwan.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 197 páginas) _b38 ilustraciones, 22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Pharmacology and Toxicology _x1940-6053
505	0		_aHigh-Throughput Techniques for DNA Methylation Profiling -- Reduced Representation Bisulfite Sequencing (RRBS) and Cell Sorting Prior to DNA Methylation Analysis in Psychiatric Disorders -- Targeted DNA Methylation Analysis Methods -- Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes -- Synthesis and Application of Cell-Permeable Metabolites for Modulating Chromatin Modifications Regulated by a-Ketoglutarate-Dependent Enzymes -- High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR -- Methods for MicroRNA Profiling in Cancer -- Microbiota and Epigenetic Regulation of Inflammatory Mediators -- Optical Microscopy and Spectroscopy for Epigenetic Modifications in Single Living Cells -- MicroRNAs in Therapy and Toxicity -- Genetics and Epigenetics of Multiple Sclerosis.
520			_aThis practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicologyseries, the book includes the kind of detail and implementation advice to encourage success in the lab. Authoritative and easily applicable, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases aims to provide pharmacologists, molecular biologists, bioinformaticians, and toxicologists with a vital background on epigenetics and state-of-the-art techniques in epigenomics.
700	1		_aStefanska, Barbara _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMacEwan, David J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967414
776	0	8	_iPrinted edition: _z9781493967421
776	0	8	_iPrinted edition: _z9781493982899
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6743-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235712 _d235712