000			03971nam a22003735i 4500
001			235713
003			ES-VaUE
005			20221220020722.0
007			cr nn 008mamaa
008			170327s2017 xxu| s |||| 0|eng d
020			_a9781493969319
024	7		_a10.1007/978-1-4939-6931-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aT-Cell Trafficking _bMethods and Protocols _cedited by George Edward Rainger, Helen M. Mcgettrick.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 252 páginas) _b47 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1591
505	0		_aIntroduction to Homeostatic Migration -- Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices -- Visualising and Tracking T Cell Motility in vivo -- Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network -- Introduction: T cell Trafficking in Inflammation and Immunity -- Leukocyte Adhesion under Haemodynamic Flow Conditions -- Endocrine Regulation of Lymphocyte Trafficking In Vitro -- Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells -- Monitoring RhoGTPase Activity in Leukocytes using Classic "pull-down" Assays -- Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions -- Introduction to Lymphocyte Trafficking in Disease -- Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking -- In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma -- Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue -- Tissue Digestion for Stromal Cell and Leukocyte Isolation -- T-cell Response in the Lung Following Influenza Virus Infection. .
520			_aThis second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during 'physiological' resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.
700	1		_aRainger, George Edward _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMcgettrick, Helen M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969296
776	0	8	_iPrinted edition: _z9781493969302
776	0	8	_iPrinted edition: _z9781493983421
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6931-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235713 _d235713