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008			100301s2003 xxu| s |||| 0|eng d
020			_a9781592594191
024	7		_a10.1385/1592594190 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBlood'Brain Barrier _bBiology and Research Protocols _cedited by Sukriti Nag.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XIV, 550 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v89
505	0		_aTissue Techniques -- Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels -- Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry -- Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions -- Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy -- Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy -- Blood-Brain Barrier Permeability Techniques -- Pathophysiology of Blood-Brain Barrier Breakdown -- Pial Vessel Permeability to Tracers Using Cranial Windows -- Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry -- Ultracytochemical Studies of the Compromised Blood-Brain Barrier -- Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier -- Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers -- Blood-Brain Barrier Transport Techniques -- A Review of Blood-Brain Barrier Transport Techniques -- In Situ Brain Perfusion Technique -- Brain Efflux Index Method -- Transport Studies Using Membrane Vesicles -- Drug Transport Studies Using Quantitative Microdialysis -- Single-Pass Dual-Label Indicator Method -- Protein Transport in Cerebral Endothelium -- Transport Across the Choroid Plexuses In Vivo and In Vitro -- In Vitro Techniques -- An Overview of In Vitro Techniques for Blood-Brain Barrier Studies -- Isolation and Characterization of Human Brain Endothelial Cells -- Human Brain Microvessel Endothelial Cell and Leukocyte Interactions -- Development and Characterization of Immortalized Cerebral Endothelial Cell Lines -- Isolation and Characterization of Retinal Endothelial Cells -- Isolation and Characterization of Cerebral Microvascular Pericytes -- Molecular Techniques -- Molecular Biology of the Blood-Brain Barrier -- Blood-Brain Barrier Genomics -- Dynamic In Vitro Model of the Blood-Brain Barrier -- Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells -- mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization -- Proteomics of Brain Endothelium -- Immunoblot Detection of Brain Vascular Proteins -- Immunohistochemical Detection of Endothelial Proteins -- Genetically Altered Mice -- Cerebral Vascular Function in Genetically Altered Mice -- Generation of Transgenic Mice by Pronuclear Injection -- Mouse Genome Modification.
520			_aDespite the fact that the breakdown of the blood-brain barrier occurs in many brain diseases and is an important factor in mortality, the molecular mechanisms involved in the breakdown are not completely understood. In The Blood-Brain Barrier: Biology and Research Protocols, leading researchers and clinician-scientists present state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium. Comprehensive and state-of-the-art, The Blood-Brain Barrier: Biology and Research Protocols offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.
700	1		_aNag, Sukriti _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373398
776	0	8	_iPrinted edition: _z9781588290731
776	0	8	_iPrinted edition: _z9781489938336
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592594190 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2003
999			_c235736 _d235736