000			02803nam a22003615i 4500
001			235738
003			ES-VaUE
005			20221220020723.0
007			cr nn 008mamaa
008			161017s2017 gw | s |||| 0|eng d
020			_a9783662529591
024	7		_a10.1007/978-3-662-52959-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aFluorescence In Situ Hybridization (FISH) _bApplication Guide _cedited by Thomas Liehr.
250			_a2nd edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (XIII, 606 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aPart I Required Equipment and Probes for FISH Procedures -- Part II FISH Procedure -- Part III Material Suited for FISH Applications in Humans -- Part IV Multicolor-FISH-Probe Sets (mFISH) and Immunostaining -- Part V Interphase FISH -- Part VI Applications of FISH in Zoology, Botany and Microbiology -- Part VII Comparative Genomic Hybridization (CGH) and Array CGH.
520			_aThis manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.
700	1		_aLiehr, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662529577
776	0	8	_iPrinted edition: _z9783662529584
776	0	8	_iPrinted edition: _z9783662571002
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-52959-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235738 _d235738