000			03311nam a22003495i 4500
001			235744
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2006 xxu| s |||| 0|eng d
020			_a9781597451543
024	7		_a10.1007/978-1-59745-154-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNuclear Transfer Protocols _bCell Reprogramming and Transgenesis _cedited by Paul J. Verma.
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XII, 338 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v348
505	0		_aNuclear Transfer -- Origin and Progress of Nuclear Transfer in Nonmammalian Animals -- In Vitro Maturation and Embryo Production in Cattle -- Activation of Oocytes After Nuclear Transfer -- Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos -- Maintenance of Pregnancy in Pigs With Limited Viable Embryos -- Health and Neonatal Care of Bovine Clones -- Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice -- Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors -- A Double Nuclear Transfer Technique for Cloning Pigs -- Nuclear Transfer in Nonhuman Primates -- Cloning of Exotic/Endangered Species: Desert Bighorn Sheep -- Handmade Somatic Cell Cloning in Cattle -- Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells -- Transgenesis Using Nuclear Transfer in Goats -- Reprogramming Nuclei -- Nuclear Reprogramming -- Expression of Imprinted Genes in Cloned Mice -- Nuclear Remodeling Assay in Xenopus Egg Extract -- In Vitro Reprogramming of Nuclei and Cells -- Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells -- Gene Expression and Methylation Patterns in Cloned Embryos -- Concluding Remarks -- Are There Any Normal Clones? -- Future and Applications of Cloning.
520			_aNuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
700	1		_aVerma, Paul J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617374685
776	0	8	_iPrinted edition: _z9781588292803
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-154-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2006
999			_c235744 _d235744