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008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592596423
024	7		_a10.1007/978-1-59259-642-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular Biomethods Handbook _cedited by Ralph Rapley, John M. Walker.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XIII, 725 páginas) _b360 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aExtraction of Total RNA from Tissues and Cultured Cells -- Isolation of Total Cellular DNA from Tissues and Cultured Cells -- Gel Electrophoresis of DNA -- Sl Nuclease Analysis of RNA -- Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA) -- Gene Probes -- Nonradioactive Labeling of DNA -- Southern Blot Analysis -- Northern Blot Analysis -- DNA Sequencing -- Autoradiography and Fluorography -- Mobility Shift Assays -- cDNA Libraries -- Genomic DNA Libraries -- ? as a Cloning Vector -- Plasmid-Derived Cloning Vectors -- M13 and Phagemid-Based Cloning Vectors -- Retroviral Vectors From Laboratory Tools to Molecular Medicines -- Baculovirus Vectors -- Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes -- Plant Transformation -- Restriction Fragment-Length Polymorphisms -- Genome Mapping -- Yeast Artificial Chromosomes -- Polymerase Chain Reaction -- In Vitro Transcriptions -- In Vitro Translation -- Site-Directed Mutagenesis -- Site-Directed Mutagenesis -- DNA Profiling -- Radiolabeling of Peptides and Proteins -- Protein Electrophoresis -- Free Zone Capillary Electrophoresis -- Protein Blotting -- Ion-Exchange Chromatography -- Size-Exclusion Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Reversed-Phase HPLC -- Glycoprotein Analysis -- Protein Sequencing -- Solid-Phase Peptide Synthesis -- Protein Engineering -- Monoclonal Antibodies -- Phage-Display Libraries -- Enzyme- Linked Immunosorbent Assay (ELISA) -- Epitope Mapping -- lmmunocytochemistry -- Flow Cytometry -- Mass Spectrometry -- The Technique of In Situ Hybridization.
520			_aIn the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWalker, John M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943552
776	0	8	_iPrinted edition: _z9781489943545
776	0	8	_iPrinted edition: _z9780896035010
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59259-642-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c235753 _d235753