000			03971nam a22003615i 4500
001			235760
003			ES-VaUE
005			20221220020724.0
007			cr nn 008mamaa
008			160419s2016 xxu| s |||| 0|eng d
020			_a9781493935697
024	7		_a10.1007/978-1-4939-3569-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aComputational Design of Ligand Binding Proteins _cedited by Barry L. Stoddard.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XVI, 375 páginas) _b94 ilustraciones, 76 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1414
505	0		_aIn silico Identification and Characterization of Protein-Ligand Binding Sites -- Computational Modeling of Small Molecule Ligand Binding Interactions and Affinities -- Binding Site Prediction of Proteins with Organic Compounds or Peptides Using GALAXY Web Servers -- Rosetta and the Design of Ligand Binding Sites -- PocketOptimizer and the Design of Ligand Binding Sites -- Proteus and the Design of Ligand Binding Sites -- A Structure Based Design Protocol for Optimizing Combinatorial Protein Libraries -- Combined and Iterative Use of Computational Design and Directed Evolution for Protein-Ligand Binding Design -- Improving Binding Affinity and Selectivity of Computationally Designed Ligand Binding Proteins Using Experiments -- Computational Design of Multinuclear Metalloproteins Using Unnatural Amino Acids -- De Novo Design of Metalloproteins and Metalloenzymes in a Three-helix Bundle -- Design of Light-Controlled Protein Conformations and Functions -- Computational Introduction of Catalytic Activity into Proteins -- Generating High Accuracy Peptide Binding Data in High Throughput with Yeast Surface Display and SORTCERY -- Design of Specific Peptide-Protein Recognition -- Computational Design of DNA-binding Proteins -- Motif-driven Design of Protein-Protein Interfaces -- Computational Reprogramming of T Cell Antigen Receptor Binding Properties -- Computational Modeling of T Cell Receptor Complexes -- Computational Design of Protein Linkers -- Modeling of Protein-RNA Complex Structures Using Computational Docking Methods.
520			_aThis volume provides a collection of protocols and approaches for the creation of novel ligand binding proteins, compiled and described by many of today's leaders in the field of protein engineering. Chapters focus on modeling protein ligand binding sites, accurate modeling of protein-ligand conformational sampling, scoring of individual docked solutions, structure-based design program such as ROSETTA, protein engineering, and additional methodological approaches. Examples of applications include the design of metal-binding proteins and light-induced ligand binding proteins, the creation of binding proteins that also display catalytic activity, and the binding of larger peptide, protein, DNA and RNA ligands. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
700	1		_aStoddard, Barry L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493935673
776	0	8	_iPrinted edition: _z9781493935680
776	0	8	_iPrinted edition: _z9781493980802
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3569-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235760 _d235760