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008			170413s2017 xxu| s |||| 0|eng d
020			_a9781493969371
024	7		_a10.1007/978-1-4939-6937-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPeroxisomes _bMethods and Protocols _cedited by Michael Schrader.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XVII, 347 páginas) _b63 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1595
505	0		_aIsolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation -- Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes -- Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays -- Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae -- Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid -- Analysis of Plasmalogen Synthesis in Cultured Cells -- Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies -- siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells -- Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals -- Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3'-diaminobenzidine -- Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins -- Detection and Immunolabelling of Peroxisomal Proteins -- Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis -- Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes -- KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress -- Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed -- In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence Microscopy -- Blue Native Page: Applications to Study Peroxisome Biogenesis -- In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes -- Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System -- The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells -- Detection of Ubiquitinated Peroxisomal Proteins in Yeast -- Assessing Pexophagy in Mammalian Cells -- Experimental Systems to Study Yeast Pexophagy -- Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells -- Study of Peroxisomal Protein Phosphorylation by Functional Proteomics -- Analysis of Peroxisomal β-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana Seedlings -- Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made Easy -- Generation of Peroxisome-Deficient Somatic Animal Cell Mutants -- Clinical and Laboratory Diagnosis of Peroxisomal Disorders.
520			_aThis volume provides easily accessible and comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Chapters detail valuable instructions, guidelines and protocols for molecular cell biologists, biochemists and biomedical researchers with an interest in peroxisome biology. Chapters in Peroxisomes: Methods and Protocols illustrate the isolation of peroxisomes, investigation of properties of membrane proteins, protocols to investigate and manipulate peroxisomes in cellular systems, detection of peroxisomes, including immunofluorescence, cytochemistry, cryo-immuno electron microscopy, and live cell imaging approaches. Authoritative and practical, Peroxisomes: Methods and Protocols aims to be useful for those already working on peroxisomes as well as for those who would like to start working on this fascinating organelle.
700	1		_aSchrader, Michael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969357
776	0	8	_iPrinted edition: _z9781493969364
776	0	8	_iPrinted edition: _z9781493983445
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6937-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235764 _d235764