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001			235767
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008			150323s2015 xxu| s |||| 0|eng d
020			_a9781493925698
024	7		_a10.1007/978-1-4939-2569-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRab GTPases _bMethods and Protocols _cedited by Guangpu Li.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 358 páginas) _b82 ilustraciones, 54 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1298
505	0		_aRab Family of GTPases -- Bioinformatic Approaches to Identifying and Classifying Rab Proteins -- Rab-NANOPS: FRET-Biosensors for Rab Membrane Nano clustering and Prenylation Detection in Mammalian Cells -- High Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins -- Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts -- Analysis of the Interactions Between Rab GTPases and Class V Myosins -- Assaying the Interaction of the Rab Guanine Nucleotide Exchange Protein Sec2 with the Upstream Rab, a Downstream Effector and a Phosphoinositide -- Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11 -- Ypt1 and TRAPP Interactions: Optimization of Multi-Color Bimolecular Fluorescence Complementation in Yeast -- Identifying a Rab Effector on the Macro autophagy Pathway -- Functional Analysis of Rab27 and its Effector Slp-2a in Renal Epithelial Cells -- Small GTPases in Acrosomal Exocytosis -- Rab Antibody Characterization: Comparison of Rab14 Antibodies -- Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin -- 3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation -- In Vitro and In Vivo Characterization of the Rab11-GAP Activity of Drosophila Evi5 -- Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance -- Measurement of Rab35 Activity by the GTP-Rab35 Trapper RBD35 -- Analysis of Connecdenn 1-3 (DENN1A-C) GEF Activity for Rab35 -- Assay of Rab17 and its Guanine Nucleotide Exchange Factor Rabex-5 in Dendrites of Hippocampal Neurons -- Methods for Analysis of AP-3/Rabin4' in Regulation of Lysosome Distribution -- Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay -- Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5 -- Role of the Rab5 Guanine Nucleotide Exchange Factor, Rme-6, in the Regulation of Clathrin-Coated Vesicle Uncoating -- Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells With or Without NGF -- Determining the Role of Rab7 in Constitutive and Ligand-Mediated Epidermal Growth Factor Receptor Endocytic Trafficking Using Single Cell Assays -- Visualizing Directional Rab7 and TrkA Co-Trafficking in Axons by pTIRF Microscopy -- Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein.
520			_aThis volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters, and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling, and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.
700	1		_aLi, Guangpu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493925704
776	0	8	_iPrinted edition: _z9781493925681
776	0	8	_iPrinted edition: _z9781493955947
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2569-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235767 _d235767