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008			100301s2008 xxu| s |||| 0|eng d
020			_a9781603274616
024	7		_a10.1007/978-1-60327-461-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Nucleus _bVolume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging _cedited by Ronald Hancock.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XVIII, 444 páginas) _b96 ilustraciones, 14 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v464
505	0		_aPhysical and Mechanical Properties -- Physical Properties of the Nucleus Studied by Micropipette Aspiration -- Mechanical Properties of Interphase Nuclei Probed by Cellular Strain Application -- Chromatin and Transcription -- Gene Expression in Polytene Nuclei -- Electron Microscope Visualization of RNA Transcription and Processing in Saccharomyces cerevisiae by Miller Chromatin Spreading -- Combing Genomic DNA for Structural and Functional Studies -- Using Molecular Beacons to Study Dispersal of mRNPs from the Gene Locus -- Mapping Cis- and Trans- Chromatin Interaction Networks Using Chromosome Conformation Capture (3C) -- Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope -- Using Cells Encapsulated in Agarose Microbeads to Analyse Nuclear Structure and Functions -- The Nuclear Envelope -- Investigation of Nuclear Envelope Structure and Passive Permeability -- Reconstitution of Nuclear Import in Permeabilized Cells -- Nuclear Envelope Formation In Vitro: A Sea Urchin Egg Cell-Free System -- Modifications of Nuclear Proteins -- Detection and Analysis of (O-linked ?-N-Acetylglucosamine)-Modified Proteins -- Detection of Sumoylated Proteins -- Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Damage -- Purification and Analysis of Variant and Modified Histones Using 2D PAGE -- Quantification of Redox Conditions in the Nucleus -- Protein Dynamics in the Nucleus -- Fluorescence Correlation Spectroscopy to Assess the Mobility of Nuclear Proteins -- Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics -- Fluorescence Recovery After Photobleaching (FRAP) to Study Nuclear Protein Dynamics in Living Cells -- Imaging Methods -- Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus -- Visualisation of RNA by Electron Microscopic In Situ Hybridisation -- Electron Spectroscopic Imaging of the Nuclear Landscape -- Cryoelectron Microscopy of Vitreous Sections: A Step Further Towards the Native State.
520			_aAlthough our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging presents biophysical approaches to study the mechanical properties of nuclei, together with a comprehensive range of imaging methods. These include FISH, FRAP, FRET, molecular beacons, fluorescence correlation spectroscopy, single molecule tracking, and combing DNA for optical microscopy, recognition imaging for atomic force microscopy, and hybridisation, tomography, and spectroscopic imaging for electron microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today.
700	1		_aHancock, Ronald _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603278874
776	0	8	_iPrinted edition: _z9781617378980
776	0	8	_iPrinted edition: _z9781603274609
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-461-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235779 _d235779