000			02035nam a22003014i 4500
001			8098
999			_c8098 _d8098
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008			200908s2020 ||||| |||| 00| 0 eng d
020			_a978-1-0716-0747-3
040			_bspa _dES-VaUE
050			_aQH588 _b.I58 2020 EB
245			_aIntestinal Stem Cells : _bMethods and Protocols _cPaloma Ordóñez-Morán (editor)
260			_aCham _aSpringer Nature _bSpringer Science+Business Media _c2020
300			_a1 recurso en línea (350 páginas)
336			_2rdacontent _aTexto (visual) _btxt
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
520			_aThis detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties. Beginning with a section on in vitro techniques to study different aspects of the intestinal stem cell functions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the isolation of intestinal crypts to generate and establish 3D organoids, as well as different animal models of gastrointestinal cancer and examples of the use of in vivo methods for studying intestinal tumor-initiating cells or cancer stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
650		7	_2embne _97767 _aCitología
650		7	_2embne _97765 _aCélulas
700			_9515730 _aOrdóñez-Morán, Paloma
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=http://link.springer.com/book/10.1007/978-1-0716-0747-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
998			_db